Enzyme Assays: A Practical ApproachRobert Eisenthal, Michael J. Danson Oxford University Press, 2002 - 282 páginas Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences. |
Contenido
III | 1 |
IV | 15 |
V | 18 |
VI | 20 |
VII | 29 |
VIII | 49 |
XI | 70 |
XII | 71 |
LVIII | 173 |
LIX | 178 |
LX | 204 |
LXI | 207 |
LXIV | 213 |
LXV | 217 |
LXVI | 218 |
LXVII | 219 |
XIII | 79 |
XV | 80 |
XVI | 97 |
XVII | 101 |
XXI | 104 |
XXII | 115 |
XXIII | 117 |
XXIV | 121 |
XXV | 123 |
XXVI | 139 |
XXIX | 140 |
XXXI | 142 |
XXXII | 143 |
XXXIII | 146 |
XXXIV | 147 |
XL | 148 |
XLI | 149 |
XLIII | 151 |
XLIV | 153 |
XLV | 155 |
XLIX | 157 |
L | 160 |
LI | 166 |
LIV | 169 |
LXVIII | 221 |
LXIX | 222 |
LXX | 223 |
LXXIII | 224 |
LXXIV | 231 |
LXXV | 233 |
LXXIX | 235 |
LXXX | 243 |
LXXXI | 244 |
LXXXIII | 245 |
LXXXV | 247 |
LXXXVIII | 249 |
LXXXIX | 250 |
XC | 256 |
XCII | 258 |
XCIII | 260 |
XCIV | 262 |
XCV | 265 |
XCVI | 266 |
XCVII | 267 |
XCVIII | 271 |
275 | |
Otras ediciones - Ver todas
Enzyme Assays: A Practical Approach Robert Eisenthal,Michael J. Danson Sin vista previa disponible - 2002 |
Términos y frases comunes
absorbance acetate acid addition Anal analysis assay mixture behaviour binding Biochem biotinylated blank rate catalysed cell Centrifuge Chem chromatography chromophore column components compounds containing cuvette dehydrogenase detection detector determined diluted EDTA effects electrode Electrode buffer Electrophoresis Electrophoresis Starch gel enzymatic enzyme activity enzyme assays enzyme concentration enzyme kinetics equation Equipment and reagents example extraction Figure fluorescence formation homogenization HPLC incubation inhibition inhibitor initial rate instrument kinase kinetic lactate dehydrogenase light linear malate dehydrogenase measured membrane methanol mg/ml MgCl2 Michaelis-Menten Michaelis-Menten equation mobile phase molecules NADH NADP NADPH oxidase oxidation parameters peptide pH-stat phosphate plate plot PMSF procedure progress curve protein proteinases Protocol range reaction mixture Reaction scheme result reverse phase sample screening sensitivity sodium solution solvent SPA beads spectrophotometer Starch staurosporine substrate substrate concentration temperature tion tissue titration Tris-HCl V/cm values velocity volume wavelength
Referencias a este libro
Biochimica industriale: Enzimi e loro applicazioni nella bioindustria R. Verga,M.S. Pilone Sin vista previa disponible - 2001 |
Biochimica industriale: Enzimi e loro applicazioni nella bioindustria R. Verga,M.S. Pilone Sin vista previa disponible - 2001 |