Enzyme assays : a practical approach
Enzyme assays are among the most frequently performed procedures in biochemistry, routinely used (for example) to estimate the amount of enzyme present in a cell or tissue. This new edition describes the design and execution of enzyme assays, covering both general principles and specific examples.
xix, 282 p. ; 24 cm.
9780199638215, 9780199638208, 0199638217, 0199638209
642434680
1. Principles of enzyme assay and kinetic studies ; 2. Photometric assays ; 3. Radiometric assays ; 4. High performance liquid chromatography assays ; 5. Electrochemical assays: the oxygen electrode ; 6. Electrochemical assays: the nitric oxide electrode ; 7. Electrochemical assays: the pH-stat ; 8. Enzyme assays after gel electrophoresis ; 9. Techniques for enzyme extraction ; 10. Determination of active site concentration ; 11. High throughput screening - considerations for enzyme assays ; 12. Statistical analysis of enzyme kinetic data